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Amylomyces rouxii Strain CBS 438.76 Affects Cholesterol Metabolism in Cholesterol-Fed Rats OAK
FUKUSHIMA, Michihiro; HAN, Kyu-Ho; TANEICHI, Yae; IIJIMA, Setsuko; SHIMADA, Ken-ichiro; SAITOH, Katsuichi; ODA, Yuji; CHIJI, Hideyuki; SEKIKAWA, Mitsuo; 福島, 道広; 韓, 圭鎬; 島田, 謙一郎; 小田, 有二; 関川, 三男.
We examined the effects of Amylomyces rouxii, which is a mold found in some fermented foods in Indonesia, on serum cholesterol and hepatic LDL receptor mRNA in rats. Rats were fed a 0.5% cholesterol-enriched diet with (A. rouxii group) or without (control group) 30g/kg A. rouxii for 4 wk. There were no significant differences in the body weight, food intake or liver weight among the groups. However, the weight of the cecum in the A. rouxii-fed group was significantly higher than that in the control group. The cecal pH in the A. rouxii-fed group was significantly lower than that in the control group. Cecal acetic acid, propionic acid and total SCFA concentrations in the A. rouxii-fed group were significantly higher than those in the control group. The serum...
Palavras-chave: Rats; Amylomyces rouxii strain CBS 438.76; Cholesterol; Cholesterol 7α-hydroxylase mRNA; LDL receptor mRNA.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2911
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Correlation between the Presence of Ubiquitin Conjugated Protein Phosphatase Inhibitor 1 and Postmortem Muscle Glycogen Metabolism OAK
NYAM-OSOR, Purevdorj; SHIMADA, Kenichiro; HAN, Kyu-Ho; FUKUSHIMA, Michihiro; SEKIKAWA, Mistuo; 島田, 謙一郎; 韓, 圭鎬; 福島, 道広; 関川, 三男.
Recently, we have identified the ubiquitin conjugate, protein phosphatase inhibitor 1 (I1), from postmortem muscles. Since I1 is one of the important regulators of glycogen metabolism, the effect of this conjugate (Ub-I1) present in postmortem muscle on glycolysis was investigated. Two sample groups were distinguishable: those not containing Ub-I1 (I, n=5)and those containing Ub-I1 (II, n=27). Due to the enormous variation in ultimate pH at 48 h postmortem, the latter group was subcategorized into a IIa group showing lower or normal pH and a IIb group showing higher or dark, firm, dry meat pH. Postmortem glycolysis occurred rapidly and vigorously among group I samples. The rate and extent of postmortem glycolysis was typical in group IIa samples while it...
Palavras-chave: Ubiquitin conjugate; Protein phosphatase inhibitor 1; Glycogen; Glycolysis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2961
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Effects of Anthocyanin-rich Colored Potato Flakes on Lipid Oxidation, Instrumental Color Evaluation and Sensory Characteristics in Cooked Pork Sausages OAK
JAYAWARDANA, Barana C.; YANAGIHARA, Maki; HAN, Kyu-Ho; FUKUSHIMA, Michihiro; SEKIKAWA, Mitsuo; SHIMADA, Kenichiro; 韓, 圭鎬; 福島, 道広; 関川, 三男; 島田, 謙一郎.
We investigated lipid oxidation, instrumental color evaluation, and sensory characteristics in cooked pork sausages containing anthocyanin rich colored potato (Solanum tuberosam cv. Shadow Queen) flakes (CPF). According to the thiobarbituric acid reactive substance values, addition of 2% CPF suppressed lipid oxidation (P < 0.05) by 80% compared to the control. The antioxidant activity of 2% CPF was comparable to the synthetic antioxidant butylated hydroxytoluene (0.1%) in cooked pork sausages. The L, a* values and odor, flavor, taste, texture and overall sensory quality did not significantly differ between control and 2% CPF. Overall, cooked pork sausage with 2% CPF was as acceptable to consumers as the control. Thus, the present study indicated that...
Palavras-chave: Anthocyanin; Colored potato flake; Lipid oxidation; Natural antioxidant; Pork sausage.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3842
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Effects of Food Additives on Susceptibility of Gram Negative Bacteria Derived from Dry-Fermented Sausage OAK
DORJ, Serjmyadag; SHIMADA, Kenichiro; SEKIKAWA, Mitsuo; 島田, 謙一郎; 関川, 三男.
This study examined the effects of food additives on gram-negative bacteria. The food additives used included synthetic antioxidants (butylated hydroxyanisole, BHA, and butylated hydroxytoluene, BHT), a curing agent and lactic acid with or without a cell-free supernatant (CFS) containing antimicrobial compounds of Lactobacillus sakei D-1001. The gram-negative bacteria were selected from dry-fermented sausages and cultured with different food additives for 18 h in nutrient broth, and then another 24 h with or without CFS adjusted (at pH 6.0) to inactivate lactic acid or not adjusted (at pH 4.0). BHA (0.1%) resulted in total viable cell inhibition following 18 h culture. A reduction in cell growth was observed in culture broths with 0.1% lactic acid and...
Palavras-chave: Food additive antimicrobial compounds; Gram-negative bacteria.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2960
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Enzymatic Hydrolysis and Ethanol Fermentation of By-Products from Potato Processing Plants OAK
YAMADA, Sunao; SHINOMIYA, Noriyuki; OHBA, Kiyoshi; SEKIKAWA, Mitsuo; ODA, Yuji; 関川, 三男; 小田, 有二.
By-products from potato processing plants were liquefied and partially saccharified using three commercially available enzymes, followed by ethanol fermentation by yeast and then hydrolysis by glucoamylase. From 12% (w/w) of fresh potato peel, about 20 mg/mL ethanol was formed in the supernatant via amylase, pectinase, or an enzyme complex; this yield was slightly increased with combinations of these enzymes. Supplementation of substandard mash to potato peel (1:1) as a raw material enhanced the amount of ethanol formation, increasing it to approximately 50 mg/mL by the mixture of all three enzymes. After the addition of yeast and glucoamylase to the partially saccharified material, ethanol formation proceeded gradually and slowed in 12 h with the...
Palavras-chave: Potato peel; Substandard mash; Ethanol; Fermentation.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2962
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Porcine artery elastin preparation reduces serum cholesterol level in rats OAK
Liyanage, Ruvini; Nakamura, Yumi; Shimada, Ken-ichiro; Sekikawa, Mitsuo; Jayawardana, Barana Chaminda; Han, Kyu-Ho; Tomoko, Okada; Ohba, Kiyoshi; Takahata, Yoshihisa; Morimatsu, Fumiki; Fukushima, Michihiro; 福島, 道広; 島田, 謙一郎; 関川, 三男; 韓, 圭鎬.
The effect of porcine artery elastin on serum cholesterol level was investigated in rats fed a cholesterol-free diet. Rats were fed for 4 weeks, with a diet (ED) containing 15% casein and 5% of porcine artery elastin in comparison with a diet (CD) containing 20% casein. The total serum and non-HDL-cholesterol concentrations were lower (P<0.001) in ED-fed group than the CD-fed group at the end of the experiment. Caecal propionate concentration and Bifidobacterium and Lactobacillus population were higher (P<0.05) in ED-fed group than the CD-fed group. The results of this study suggest that porcine artery elastin could be considered as a functional dietary protein with hypocholesterolaemic ability. Favourable amino acid composition and lysine derived...
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2681
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Potato Pulps Lowered the Serum Cholesterol and Triglyceride Levels in Rats OAK
HASHIMOTO, Naoto; ITO, Yusaku; HAN, Kyu-Ho; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo; TOPPING, David L.; BIRD, Anthony R.; NODA, Takahiro; CHIJI, Hideyuki; FUKUSHIMA, Michihiro; 韓, 圭鎬; 島田, 謙一郎; 関川, 三男; 福島, 道広.
日本ビタミン学会、日本栄養・食糧学会共同編集
Palavras-chave: Cholesterol; Triglyceride; Potato pulps; Liver.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2910
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Preparation method modulates hypocholesterolaemic responses of potato peptides OAK
Liyanage, Ruvini; Minamino, Saori; Nakamura, Yumi; Shimada, Ken-ichiro; Sekikawa, Mitsuo; Sasaki, Keiko; Ohba, Kiyoshi; Jayawardana, Barana Chaminda; Shibayama, Shin-ichi; Fukushima, Michihiro; 福島, 道広; 島田, 謙一郎; 関川, 三男.
In this study we compared the hypocholesterolaemic ability of two potato peptide preparations in rats. Experimental groups were fed for 4 weeks, with casein as the basal diet, in comparison with two diets containing 20% potato peptide preparations PPS (Short hydrolysis preparation) and PPL (Long hydrolysis preparation). Serum total cholesterol and serum triglyceride level were lower in PPS-fed group compared with CN-and PPL-fed groups. Lower non-HDL cholesterol level (P<0.05) in both PPS-and PPL-fed groups, was followed by higher neutral sterol excretion, and higher hepatic LDL-R and SR-B1 mRNA level than the control. Hepatic SREBP-2 and HMG-CoA reductase mRNA level were higher in PPL-fed group compared with the CN-fed group (P<0.05). Caecal total...
Palavras-chave: Potato peptide; Cholesterol; Short chain fatty acid; Faecal steroid; Hepatic gene; Rat.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2682
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Principal component analysis of physicohemical measures of bovine Longissimus thorasis OAK
関川, 三男; 三上, 正幸; 三浦, 弘之; Sekikawa, Mitsuo; Mikami, Masayuki; Miura, Hiroyuki.
Palavras-chave: 牛肉; 肉質; 理化学的分析値; 色調; 主成分分析.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1687
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Production of Dry-cured Ham and its Microbial and Physico-Chemical Properties OAK
三上, 正幸; 島田, 謙一郎; 関川, 三男.
乾塩法により長期熟成タイプの骨付き生ハムを製造した.塩漬は9月下旬から始め,その後乾燥,洗浄,脂肪塗布を行い1年,1.5年および2年間熟成したものについて,微生物学的,理化学的性質および官能特性について調べた.表面の微生物検査では,一般生菌数および乳酸菌数が最大で1.8〜5.6×102cfu/cm2であったが,ほとんどは30以下または0であった.大腸菌群はすべて陰性,真菌類は最大で3.6×102cfu/cm2,他は30以下または0であった.ハムの内部は4ヵ所の筋肉部位について分析した.その結果,細菌数はすべての部位で300以下または0であった.pHは5.7〜5.9,食塩濃度は7.9〜9.6%,水分活性は0.83〜0.87,水分含量は51.7〜61.7%,亜硝酸根は0.1〜0.3ppmであった.ペプチド量と総遊離アミノ酸量は熟成とともに増加し,2年間熟成したものが最高値を示し,それぞれ100g当たり2,278.6mgおよび3,913.2mgであった.個々の遊離アミノ酸では,Lysがもっとも多く,次いでGlu, Leu, Ala, Argの順であった.官能評価では色調が高く,匂いは低い傾向が認められた.なお,総合評価では1.5年間熟成のものが高い値であった.
Palavras-chave: Dry-cured ham; Non-heated meat products.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2602
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Tartary Buckwheat Sprout Powder Lowers Plasma Cholesterol Level in Rats OAK
KUWABARA, Tomoko; HAN, Kyu-Ho; HASHIMOTO, Naoto; YAMAUCHI, Hiroaki; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo; FUKUSHIMA, Michihiro; 島田, 謙一郎; 関川, 三男; 福島, 道広; 韓, 圭鎬.
日本ビタミン学会、日本栄養・食糧学会共同編集
Palavras-chave: Bile acid; Buckwheat sprout powder; Hepatic mRNAs; Serum cholesterol; Short-chain fatty acid.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2909
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グラム陰性桿菌に対するグリシンの抑菌作用 OAK
三浦, 弘之; 関川, 三男; MIURA, Hiroyuki; SEKIKAWA, Mitsuo.
http://www.obihiro.ac.jp/~library/kenkyu.html
Ano: 1979 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3715
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チーズホエーを給与した豚の肉質特性 OAK
三上, 正幸; 島田, 謙一郎; 関川, 三男; 福島, 道広; 齋藤, 愛; 柴田, 政二; MlKAMI, Masayuki; SHIMADA, Ken-ichiro; SEKlKAWA, Mitsuo; FUKUSHIMA, Michihiro; SAITOH, Ai; SHIBATA, Seiji.
Thirty eight pigs born from same sow were divided into 2 group,and fed with or without cheese whey. Pigs were slaughtered after 137-172 days when the live weight was nearly 110kg. Samples of M. longissimus thoracis were analyzed for physicochemical properties. There was no significant difference in the pH,water content and colour appearance in pork between the whey and control group. Crude protein content of the whey group (21.7%) was significantly lower than that of the control (22.6%). Drip loss was measured at 1,5 and 10 days after slaughter,and the drip loss measured at 10 days was significantly lower in the whey group (25.4%) than that of the control (27.1%). Cooking loss was measured at 5 days after slaughter,was significantly lower in the whey group...
Palavras-chave: 豚肉; チーズホエー; ホエー; クッキングロス; ドリップロス; Pork; Cheese whey; Whey; Cooking loss; Drip loss.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3090
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ヨーロッパの非加熱食肉製品の特性について OAK
三上, 正幸; 島田, 謙一郎; 関川, 三男; MIKAMI, Masayuki; SHIMADA, Ken-ichiro; SEKIKAWA, Mitsuo.
Some properties of the non-heated meat products in European countries were analyzed. The dry fermented sausages used were Spanish salami (small,medium and large size),Chorizo, Pepper salami, Italian salami,Hungarian salami and Serrano ham. Viable bacterial counts were small number (10³-10⁵CFU/g) in the hard type sausages and were large number (10⁷ -10⁸CFU/g) in the semi soft type sausages. The pH of the dry fermented sausages inoculated with mold was 5.4-6.7 and was higher than 5.1 of Chorizo which was not inoculated with mold. Peptides and total free amino acids content were 634.0 - 2,240.7 mg/100g products and 404.8 - 2,413.4 mg/100g products, respectively. These were high numbers in Serrano ham. Nitrite ion contents were low level (0.3-4.3 ppm) in...
Palavras-chave: 発酵ソーセージ; 乾塩ハム; ペプチド; 遊離アミノ酸; Dry fermented sausages; Dry cured ham; Peptides; Free amino acids.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2992
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乳酸生成糸状菌を利用したサイレージの評価 OAK
日高, 智; 山岸, 則夫; 河合, 正人; 福島, 道広; 谷, 昌幸; 関川, 三男; 仙北谷, 康; 岡本, 明治.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1808
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乾塩ハム(ウリカリップハム)の製造とその性質 OAK
三上, 正幸; 志賀, いつか; 湊, 美子; 島田, 謙一郎; 関川, 三男.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4238
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個体死に伴う細胞内タンパク質分解系の変化 : 特に筋細胞内ユビキチン系について OAK
関川, 三男; Sekikawa, Mitsuo.
平成10年度~平成11年度科学研究費補助金 (基盤研究(C)(1))研究成果報告書(科研報告書)
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2290
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冷蔵期間が牛筋漿タンパク質画分の抽出性におよぼす影響 OAK
関川, 三男.
Ano: 1991 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/534
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反芻動物ラクトフェリンの末端領域アミノ酸配列の類似性について OAK
関川, 三男; 島崎, 敬一; 三上, 正幸; 三浦, 弘之; SEKIKAWA, Mitsuo; SHIMAZAKI, Kei-ichi; MIKAMI, Masayuki; MIURA, Hiroyuki.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: ラクトフェリン; N末端アミノ酸配列; 反芻動物.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3748
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帯広畜産大学畜産科学科畜肉保蔵学研究室における研究成果 OAK
三上, 正幸; 関川, 三男; 島田, 謙一郎.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4239
Registros recuperados: 33
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